Halo
Halo
Inspired by ohio valley style pizza
I like to think that I’m a better critical thinker than most, but I fell for the initial news story about her being trans or intersex and the fight being unfair. Then I saw the pictures of her over the years and as a kid, and I dug deeper into what actually happened and I honestly feel dirty. I’ve since been unsubbing to a lot YouTubers.
An 18 year old can decide their own fate here, hopefully they don’t have a kid of their own yet
Come visit rural Minnesota
Minnesota is as much as a toss up as Alabama
I’ve been buying used 12tb HGST from Amazon for $80.
Someone in my group chat poster the picture and I can’t unsee it. It’s horrible.
Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.
A partial solution is to remove nimby laws that prevent accessory dwelling units, duplexes, and medium and high density housing. Then you use the tax laws to heavily punish corporate ownership of low and medium density housing, and make it progressively more expensive for mom and pop landlords after a reasonable number of properties.
Hopefully this would lower the cost for single family housing, while increasing supply and variety of rentals. But somehow you’d have to prevent end stage capitalist collusion from fixing prices so competition could actually work to drive down prices.
Lol yeh but it’s partially funded by money saved from canceled subscriptions.
I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.
Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.
For what is possibly wrong with the top gel.
-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.
Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.
I paid $1000 for books my first semester of college back in 2007. I felt so burnt and violated I never bought another textbook. I made it through the rest of undergrad, a masters, and a PhD in biochemistry by checking out books from the library, borrowing textbooks from friends, and going sailing. When I taught I made it a point to teach my students about all the ways they can avoid becoming a victim like myself.
The US agent plot line was the best part of the show, and his story arc made sense. The I feel like the flag smasher plot line kinda went off the rails, and they wanted to make them sympathetic characters but lost the plot in the process. Zemo on the airplane was both entertaining and a little cringe. But the police officer stepping between falcon and the Winter soldier was so cringe I had to pause for a moment, like how does someone in America after endgame not recognize one of two avengers standing in front of him, when one has a full metallic arm. I think I would have liked it better if I wasn’t hoping for a 24 or NCIS with superheroes.
We actually both enjoyed most of she hulk, it was the ending going off the rails that was too much for my wife. She had no idea who Kevin was and all the other stuff didn’t really pay off. I’ve been sour on the MCU since falcon and the Winter soldier cringe fest, but I keep watching the movies because I would find someone enjoyment to balance out the frustration of missed opportunities to adapt old comics storylines.
For my case we have been family sharing subscriptions and Netflix was always our responsibility even though I’m well versed in the skill set needed to sail the seas, and for years it worked out great. But the family sharing nonsense has finally made it inconvenient enough to go back to the sea. Cocomelon and love is blind show on Netflix a must have for the family.
Netflix has been such a staple for so long it took a full Arr suite to get my wife to be ok that I cancelled it. When it came to Disney plus, she didn’t care because she’s not into Star wars and she hulk soured her on marvel. My 5 year old niece and nephew only ask to watch Netflix when they come over. As someone who has witnessed them kill so many franchises I love, I think Disney cracking down on password sharing is a great idea.
Me: Everytime I got time on the confocal